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WSU scientist’s species discovery helps save colorful peony from ugly disease

 

Student researcher Andrea Garfinkel chats with a farm worker in a Netherlands peony field.
Student researcher Andrea Garfinkel, right, chats with a farm worker in a Netherlands peony field during a 2015 visit. For the past three years, Garfinkel has researched diseases of peonies, discovering multiple new species of the Botrytis pathogen.

A symbol of good fortune and happy marriage, the peony has been a beloved centerpiece of floral arrangements and wedding bouquets for hundreds of years.

Pacific Northwest farmers have found success growing these bountiful blooms for a thriving global market. But they’re held back by a devastating disease: a fungus called Botrytis.

Stippling the peony’s emerald leaves with unsightly blotches of brown and purple, Botrytis can ruin up to half of growers’ crops long before they’re ready to sell.

What’s worse, the fungus can strike invisibly, turning a shipped box of seemingly perfect flowers into a brown-streaked mess, leading to upset customers and cancelled payments.

“Diseases caused by Botrytis are the peony’s number one health problem,” said Andrea Garfinkel, a fall 2017 graduate from Washington State University’s Department of Plant Pathology.

Garfinkel is helping farmers put a stop to this pervasive pest. Working with growers and scientists in the United States and Europe, she has discovered that there is more to this disease than anyone ever suspected.

Surveying 12 U.S. states using modern molecular genetics tools, Garfinkel discovered multiple new species of Botrytis, including one new species that affects grapes and peonies. She also found five other fungal diseases that had never been reported in the United States before.

“Additional knowledge about diseases that impact peonies, such as Botrytis, allows for more efficient and effective use of costly sprays, while ensuring bigger, healthier peony crops,” Garfinkel said. “That’s a win for both peony farmers and consumers.”

Garfinkel talks with growers about the health of their peonies during a 2016 Alaska field visit.
Garfinkel talks with growers about the health of their peonies during a 2016 Alaska field visit.

Answers for a growing industry

“When you study an old disease but apply new technology to it, you come up with new answers,” says Patricia Holloway, emeritus horticulture professor at the University of Alaska-Fairbanks and member of Garfinkel’s research committee.

For more than 15 years, Holloway has worked with Alaska farmers to build a successful export peony industry.

But, as Alaskan farmers cleared forests to put in peony fields, Botrytis has plagued them “almost from day one,” Holloway said. Affecting every part of cultivation, from planting to harvest and storage, Botrytis is expensive and labor intensive to control.

Holloway turned to WSU Professor Gary Chastagner, an expert on Botrytis in ornamental crops. Garfinkel, one of Chastagner’s doctoral students, accepted the challenge to find out what was happening to peonies in Alaska and other western states.

Garfinkel samples peonies in a field.
Garfinkel samples peonies in a Netherlands field. She has added nearly a third to the known species of Botrytis, a devastating disease of the popular ornamental.

Farm visits to collect DNA

Over the last three years, Garfinkel worked with Holloway and Chastagner to visit growers in Alaska, Washington and Oregon. The researchers sampled diseased specimens at planting time, during the growing season, and when plants went dormant.

After collecting samples from the blemished foliage and flowers, Garfinkel cultured the fungus, extracted its DNA, then used a process called polymerase chain reaction, or PCR, to find the code for its genes. With that code, Garfinkel could compare it with other samples of fungi to see how they are related. That was how she revealed several new species.

“Correct identification is the first step to managing any disease,” said Garfinkel. “It’s just like when you’re sick, and if you use an antibiotic on a viral infection; it doesn’t work. If growers don’t know what’s really hurting their plants, they could be doing a lot of work and spending a lot of money for nothing.”

The first of her reported discoveries, a new species called Botrytis euroamericana, found jointly with Italian scientists, was detailed earlier this year in the journal Mycologia. Garfinkel also revealed, for the first time in the Northern Hemisphere on peonies, a fungus called Mycocentrospora acerina, found only recently for the first time on peonies in Chile.

“Andrea has opened up a lot of eyes,” Holloway said. “She’s added almost one third as many names to our list of known species.

“Her work has shown us that the Botrytis disease problem is far more complex than any of us thought,” she added. “Andrea’s knowledge could make management more precise and economical, and educate peony growers worldwide.”

Contact: Andrea Garfinkel, WSU Plant Pathology doctoral graduate, andrea.garfinkel@wsu.edu